Journal: Immunology and Cell Biology

Article Title: The vaccinia‐based Sementis Copenhagen Vector coronavirus disease 2019 vaccine induces broad and durable cellular and humoral immune responses

doi: 10.1111/imcb.12539

Figure Lengend Snippet: Spike‐specific antibody and SARS‐CoV‐2 neutralization responses following SCV‐S vaccination. (a) S1 and S2 subunit endpoint IgM and IgG ELISA titers determined from serum of female C57BL/6J mice ( n = 3) at the indicated times after a single vaccination with 10 7 PFU of SCV‐S, with (b) ratio of S1‐ and S2‐specific IgG2c to IgG1 endpoint ELISA titers determined 28 days after vaccination. (c) S1 IgG ELISA binding titers (left panel) and cPass neutralization titers (right panel) in outbred ARC(s) and inbred C57BL/6J female mice ( n = 5) 21 days after a single vaccination with 10 7 PFU of SCV‐S or vector control, with (d) durability of response in the outbred cohort shown by S1‐specific endpoint IgG ELISA titers (left panel) and neutralization titers (right panel) at the indicated times. (e) S1‐specific IgG ELISA (left panel) and neutralization titers (right panel) 50 days after a single‐dose (day 0) or prime‐boost (day 0 and 28) vaccination of female C57BL/6J mice ( n = 5) with 10 7 PFU SCV‐S or control vector, with (f) neutralizing activity in ACE2 and RBD blocking ELISA, and (g) neutralizing activity (IC 80 titers) against lenti‐SARS‐CoV‐2‐S pseudoviruses bearing spike protein from the Wuhan reference strain, the alpha or beta variant, shown for prime‐boost samples. (h) Neutralization titers in young (6–8 weeks old; n = 5) and middle‐aged (9–10 months old; n = 10) C57BL/6J mice at the indicated times after prime‐boost vaccination with 10 7 PFU SCV‐S. Results shown are representative of four independent experiments (indicated above) with binding and neutralizing antibody levels comparable at similar doses and time points across all experiments. Symbols represent individual mice and bars show the mean ± s.e.m. from independent experiments. Data were log transformed and statistical significance determined using Brown–Forsythe and Welch ANOVA with Dunnett T3 multiple comparison test. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. ACE2, angiotensin‐converting enzyme‐2; IC 80 , 80% inhibitory concentration; Ig, immunoglobulin; ns, not significant; PFU, plaque‐forming units; RBD, receptor‐binding domain; SARS‐CoV‐2, severe acute respiratory syndrome coronavirus 2; SCV‐S, Sementis Copenhagen Vector spike protein.

Article Snippet: ACE2‐HEK293 recombinant cell line (catalog number 79951; BPS Bioscience, San Diego, CA, USA) was maintained in Dulbecco’s Modified Eagle Medium supplemented (Sigma Aldrich, MO, USA) with 10% fetal bovine serum, 2 mM l ‐glutamine and penicillin–streptomycin.

Techniques: Neutralization, Enzyme-linked Immunosorbent Assay, Binding Assay, Plasmid Preparation, Activity Assay, Blocking Assay, Variant Assay, Transformation Assay, Concentration Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: Examples of pyran-containing compounds with antibacterial , antioxidant and anticancer activities via inhibition of CDK2 [ , , ].

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Inhibition

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: Redocked (yellow) and co-crystallized (baby blue) ligand ( DTQ ) in the ATP binding pocket of CDK2 after self-docking calculations.

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: Binding free energy of 4d , 4k , 4f , BMS-265246 and DTQ at the active site of CDK2.

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: Three-dimensional binding interactions of DTQ within the ATP binding pocket of CDK2. Hydrogen bonds (yellow dotted lines), hydrogen atoms (white), nitrogen atoms (blue), and oxygen atoms (red).

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: Two-dimensional binding interactions of DTQ within the ATP binding pocket of CDK2.

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: Three-dimensional binding interactions of BMS-265246 within the ATP binding pocket of CDK2. Hydrogen bond (purple dotted line), hydrogen atoms (white), nitrogen atoms (blue), fluorine atoms (light green), and oxygen atoms (red).

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: Two-dimensional interactions of BMS-265246 within the ATP binding pocket of CDK2 kinase.

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: Three-dimensional model of binding interactions of compound 4d after docking calculations in the ATP binding pocket of CDK2. Hydrogen bond (black lines), hydrogen atoms (white), nitrogen atoms (blue), and oxygen atoms (red).

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: Two-dimensional model of binding interactions of compound 4d after docking calculations in the ATP binding pocket of CDK2.

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: Three-dimensional model of binding interactions of compound 4K after docking calculations in the ATP binding pocket of CDK2. Hydrogen bond (black lines), hydrogen atoms (white), nitrogen atoms (blue), and oxygen atoms (red).

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: Two-dimensional model of binding interactions of compound 4K after docking calculations in the ATP binding pocket of CDK2.

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: Three-dimensional model of binding interactions of compound 4f after docking calculations in the ATP binding pocket of CDK2. Hydrogen atoms (white), nitrogen atoms (blue), chlorine atom (green), and oxygen atoms (red).

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: Two-dimensional model of binding interactions of compound 4f after docking calculations in the ATP binding pocket of CDK2.

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: In vitro evaluation of the CDK2 inhibitory efficiency of pyrans 4d and 4K as compared to the reference inhibitor BMS-265246 over a concentration range of 0.01−10 µM. Results were obtained from three independent experiments.

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: In Vitro, Concentration Assay

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: IC 50 values of 4d , 4K and BMS-265246 against CDK2.

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques:

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: In vitro quantitative determination of the concentrations of CDK2 (ng/mL) in HCT116 cells treated with pyrans 4d and 4K compared with the positive control BMS-265246 and the negative control samples.

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: In Vitro, Positive Control, Negative Control

Journal: Pharmaceuticals

Article Title: Synthesis and Evaluation of Some New 4 H -Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations

doi: 10.3390/ph15070891

Figure Lengend Snippet: The expression profiles of the CDK2 gene in the lysate of HCT-116 cancer cells treated with compounds 4d and 4 k .

Article Snippet: CDK-2 inhibition activity of the studied compounds 4d and 4k was evaluated using a commercial CDK2 ELISA Kit (cat. no.: 79599; BPS Biosciences, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Expressing